Eskisehir Technical University Info Package Eskisehir Technical University Info Package
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About the Program Educational Objectives Key Learning Outcomes Course Structure Diagram with Credits Field Qualifications Matrix of Course& Program Qualifications Matrix of Program Outcomes&Field Qualifications
  • Graduate School of Sciences
  • Department of Advanced Technologies
  • Master of Science (MS) Degree
  • Program in Biotechnology
  • Course Structure Diagram with Credits
  • Biotechnology Laboratory Techniques II
  • Description
  • Description
  • Learning Outcomes
  • Course's Contribution to Prog.
  • Learning Outcomes & Program Qualifications

Course Introduction Information

Code - Course Title İTB504 - Biotechnology Laboratory Techniques II
Course Type Required Courses
Language of Instruction Türkçe
Laboratory + Practice 3+0
ECTS 7.5
Course Instructor(s)
Mode of Delivery face to face
Prerequisites There is no prerequisite or co-requisite for this course.
Courses Recomended -
Recommended Reading List All biotechnology and molecular biology books
Assessment methods and criteria midterm exam and final exam
Work Placement N/A
Sustainability Development Goals

Content

Weeks Topics
Week - 1 Microbial biotechnology (fermentation and biotransformation)
Week - 2 Microbial biotechnology (fermentation and biotransformation)
Week - 3 Molecular diagnostic methods (ribotyping and analyses of fatty acid methyl esters)
Week - 4 pulsed field gel electrophoresis (PFGE)
Week - 5 cell culture techniques
Week - 6 Protein analysing techniques
Week - 7 SDS-PAGE Protein techniques
Week - 8 SDS-PAGE Protein techniques
Week - 9 Basic Techniques Used for Recombinant DNA (DNA isolation from bacterium, animal and plant tissue, agorose gel electrophoresis, DNA resctriction with REs)
Week - 10 Basic Techniques Used for Recombinant DNA (DNA isolation from bacterium, animal and plant tissue, agorose gel electrophoresis, DNA resctriction with REs)
Week - 11 ligation, transformation, cDNA synthesis
Week - 12 The Techniques used in Ligation, Transformation, cDNA Synthesis.
Week - 13 Gene Cloning Techniques (mapbased cloning, expressed sequence tagging method, transposon tagging method)
Week - 14 Gene Transfer in Plants (Agrobacterium-mediated Gene Transfer, Direct Gene Transfer Methods, Particle Bombardment, Polyethylene Glycol Gene-mediated Transformation, Electroporation, Silicon Carbide Fiber-mediated Transformation)

Learning Activities and Teaching Methods

  • Teaching Methods
  • Lecture
  • Question & Answer
  • Observation
  • Field Trip
  • Team/Group Work
  • Demonstration
  • Drill - Practise
  • Brain Storming
  • Report Preparation and/or Presentation
  • Role Playing/Dramatization
  • Competences
  • Follow ethical and moral rules
  • Civic awareness
  • Environmental awareness

Assessment Methods

Assessment Method and Passing Requirements
Quamtity Percentage (%)
Toplam (%) 0
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