Akademik Dersler - Tanıtım


Code - Course Title	BİY471 - Molecular Microbial Ecology
Course Type	Area Elective Courses
Language of Instruction	Türkçe
Laboratory + Practice	0+3
ECTS	5.0
Course Instructor(s)	PROFESÖR DOKTOR MEHMET BURÇİN MUTLU
Mode of Delivery
Prerequisites	Mikrobiyoloji I ve Mikrobiyoloji II
Courses Recomended	-
Required or Recommended Resources	Molecular Microbial Ecology Manual, Editors:G. A. Kowalchuk, F. J. Bruijn, I. M. Head, A. D. Akkermans, J. D. Elsas, Hardcover ISBN 978-1-4020-2176-3 Published: 09 August 2004, Springer Dordrecht
Recommended Reading List	Molecular Microbial Ecology (Osborn & Smith), Environmental Microbiology: From Genomes to Biogeochemistry (Eugene L. Madsen),
Assessment methods and criteria
Work Placement
Sustainability Development Goals	Health and Quality of Life , Quality Education , Clean Water and Sanitation

Weeks	Topics
Week - 1	Introduction to Microbial Ecology: History, basic concepts and limitations of traditional methods (culture).
Week - 2	Diversity and Phylogeny of Microorganisms: Three Kingdom system (Archaea, Bacteria, Eukarya) and molecular markers (16S rRNA, 18S rRNA, ITS).
Week - 3	Nucleic Acid Extraction from Environmental Samples: DNA and RNA isolation techniques, purification and quality control processes.
Week - 4	Nucleic Acid Extraction from Environmental Samples: DNA and RNA isolation techniques, purification and quality control processes.
Week - 5	Polymerase Chain Reaction (PCR) Based Approaches: qPCR (quantitative PCR), RT-PCR and microbial fingerprint techniques (DGGE, T-RFLP)
Week - 6	Polymerase Chain Reaction (PCR) Based Approaches: qPCR (quantitative PCR), RT-PCR and microbial fingerprint techniques (DGGE, T-RFLP)
Week - 7	Polymerase Chain Reaction (PCR) Based Approaches: qPCR (quantitative PCR), RT-PCR and microbial fingerprint techniques (DGGE, T-RFLP)
Week - 8	Clone libraries
Week - 9	Clone libraries
Week - 10	Fluorescein in situ hybridization
Week - 11	DNA seğuencing analysis
Week - 12	DNA sequencing analysis
Week - 13	Metagenomics
Week - 14	Metagenomics

Assessment Method and Passing Requirements
	Quamtity	Percentage (%)
Toplam (%)		0